Chemical pulldown - sample processing, fractionation, protein identification and quantitation All aspects of sample processing, TMT labelling, fractionation by HPLC and LC–MS/MS, and protein identification and quantitation were described previously (Milne, R., et al., Toolkit of Approaches To Support Target-Focused Drug Discovery for Plasmodium falciparum Lysyl tRNA Synthetase. ACS Infect Dis, 2022. 8(9): p. 1962–1974). In this instance, samples were separated into 10 fractions.