This study applied the LFQ proteome to infer molecular targets and protein–ligand interactions of candidate nematocidal compounds in Haemonchus contortus. Protein extracts were prepared under standardised conditions, and then processed for quantitative proteomics using high-resolution LC-MS/MS. Resultant data sets were analysed via MaxQuant and Perseus, with protein identifications referenced to the annotated chromosome-scale genome of H. contortus Haecon-5.