Isothermal TPP was performed essentially as previously described (Milne, R., et al., Toolkit of Approaches To Support Target-Focused Drug Discovery for Plasmodium falciparum Lysyl tRNA Synthetase. ACS Infect Dis, 2022. 8(9): p. 1962–1974). In this instance P. falciparum lysates incubated ± drug were incubated at 37 and 43°C.