The skin tissues were suspended in lysis buffer (1% sodium deoxycholate (SDS) and 8 M urea) supplemented with an appropriate 1× protease inhibitor cocktail to inhibit protease activity. For sample preparation, proteins were subjected to denaturation, reduction, and alkylation as well as trypsin digestion and peptide cleanup. The direct data-independent acquisition (DIA) data were processed and analyzed by Spectronaut 18 (Biognosys AG, Switzerland) with the default settings.