This project aims to systematically characterize the proteome of oocytes predominantly from primordial follicles using an in vivo APEX-based proximity labeling strategy. Due to the rarity of primordial follicle oocytes and technical limitations in their isolation, comprehensive proteomic profiling of early-stage oocytes has remained largely unexplored. In this study, we generated transgenic APEX fluorescent mice to enable oocyte-specific protein labeling under physiological conditions. Using this system, we identified 2,772 proteins and performed quantitative analyses of proteomic alterations following short-term cisplatin exposure. The data revealed dynamic changes in pathways related to DNA damage response and histone modification. This dataset provides a valuable resource for understanding early-stage oocyte biology and chemotherapy-induced reproductive toxicity.