The experimental design included Calu-1 and H460 cells subjected to four treatment groups (Myr, YnMyr, YnMyr + 10 nM IMP-1088, YnMyr + 100 nM IMP-1088), each with four biological replicates. All samples were ligated to AzDB and affinity enriched. Following digestion, all peptides were subjected to LC-MS/MS analysis and quantified by LFQ. For the AzDB reagent, LysC and trypsin digestion first release unmodified peptides(other peptides from myristoylated proteins or background peptides). Subsequently, myristoylated peptides remaining on the agarose resin are released using formic acid, allowing the two peptide fractions to be detected separately.