To validate these findings in vivo and explore the therapeutic potential of Rab32, we employed an adeno-associated virus serotype 8 (AAV8) vector to specifically overexpress Rab32 in the livers of mice , Mice received intravenous injection of AAV-Rab32 (AAV-Rab32 group) or AAV-Con (control group), and were fed a high-fat diet (HFD)for 12 weeks simultaneously