To elucidate the composition of transcriptional complexes involving Nfkb1 in the context of abdominal aortic aneurysm (AAA), we performed co-immunoprecipitation followed by mass spectrometry (Co-IP MS) using an anti-Nfkb1 antibody in murine vascular adventitial fibroblasts overexpressing Nfkb1. Three biological replicates were analyzed (IP-1, IP-2, and IP-3). The goal was to identify protein components that physically interact with Nfkb1, potentially constituting transcriptional co-regulators within the nuclear environment.