This project focuses on the core research direction of \"Wuweizi phenol exerting multimodal antidepressant effects by regulating 5-lipoxygenase (5-LOX), inhibiting neuroinflammation, and repairing synaptic function\". It employs quantitative proteomics technology based on liquid chromatography-tandem mass spectrometry (LC-MS/MS), using a mouse model of depression-like behavior induced by chronic social defeat stress (Chronic Social Defeat Stress, CSDS) as the research object. The project systematically analyzes the differences in protein expression profiles in the prefrontal cortex (Prefrontal Cortex, PFC) of mice in the model group, normal control group, and Wuweizi phenol intervention group. The core goal is to screen key differentially expressed proteins (Differentially Expressed Proteins, DEPs) associated with CSDS-induced depression-like behaviors, 5-LOX signaling pathway regulation, neuroinflammation, and synaptic damage/repair. It aims to deeply explore the core signaling pathways and molecular regulatory networks involved in DEPs, clarify the protein-level mechanism by which Wuweizi phenol exerts antidepressant effects, and simultaneously provide solid proteomic data support and scientific theoretical basis for elucidating the pathogenesis of depression, screening potential diagnostic markers, and developing antidepressant drugs targeting 5-LOX regulation, neuroinflammation inhibition, or synaptic repair.