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PXD074331

PXD074331 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification of STAT6 Phosphorylation
DescriptionTo identify potential phosphorylation sites of STAT6, RAW 264.7 cells were overexpressed with STAT6-HA. 48 hours after transfection, cells were treated with 10 μM etoposide for 2 h, then lysed for immunoprecipitation using anti-HA beads. Immunoprecipitates were separated by SDS–PAGE and then Coomassie blue staining was carried out. Bands of interest were cut from the gel and then in-gel digestion was performed using sequencing grade-modified trypsin. The peptides were analyzed by liquid chromatography–tandem mass spectrometry (LC–MS/MS) with nano-LC combined with Orbitrap Q Exactive mass spectrometer
HostingRepositoryiProX
AnnounceDate2026-02-11
AnnouncementXMLSubmission_2026-02-10_23:42:54.668.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterZhao Zhou
SpeciesList scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationListphosphorylated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02026-02-10 23:41:40ID requested
12026-02-10 23:42:55announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: macrophage, STAT6
Contact List
Yifu Qiu
contact affiliationPeking University
contact emailyifu.qiu@pku.edu.cn
lab head
Zhao Zhou
contact affiliationPeking University
contact email874387094@qq.com
dataset submitter
Full Dataset Link List
iProX dataset URI