Project Description: TMT-Based Quantitative Proteomic Profying of SCC-9 Cells Treated with 18β-Glycyrrhetinic Acid This project employs Tandem Mass Tag (TMT) multiplexed quantitative proteomics to comprehensively analyze changes in the global protein expression profile of human tongue squamous cell carcinoma (SCC-9) cells following treatment with the bioactive compound 18β-Glycyrrhetinic Acid (18β-GA). The experiment was designed to compare the proteomes of SCC-9 cells exposed to a physiologically relevant concentration of 90uM 18β-GA against the negative control (NC) wells received medium containing 0.05% DMSO for 48h.After the intervention period, cells were harvested, and proteins were extracted, digested, and labeled using a TMT reagent kit. The pooled, labeled peptides were then fractionated and analyzed by liquid chromatography coupled to high-resolution tandem mass spectrometry (LC-MS/MS). The primary goal of this study is to identify differentially expressed proteins induced by 18β-GA, which may reveal its molecular mechanisms of action in oral cancer cells. This includes potential effects on key signaling pathways, cellular processes such as proliferation, apoptosis, migration, and metabolism, and the discovery of novel protein targets. The generated dataset provides a system-wide view of the protein-level response to 18β-GA, serving as a valuable resource for hypothesis generation and further functional validation in cancer research.