PXD074164 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Identification of MELK-mediated Cofilin-1 T70 phosphorylation by IP-LC-MS/MS analysis |
| Description | To investigate whether MELK phosphorylates Cofilin-1 at residue Threonine 70 (T70), we performed a phosphoproteomic validation using immunoprecipitation coupled with mass spectrometry (IP-LC-MS/MS). HEK293T cells were divided into three experimental groups: (1) MOCK control (Empty Vector), (2) MELK overexpression, and (3) MELK overexpression treated with the inhibitor OTSSP167. Endogenous Cofilin-1 was enriched by immunoprecipitation. The study aimed to identify and quantify the phosphorylation status of Cofilin-1, specifically focusing on the T70 site, and to assess the impact of MELK kinase activity and its inhibition by OTSSP167 on this phosphorylation event. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-04-01 |
| AnnouncementXML | Submission_2026-04-01_01:00:32.893.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Kuai Yu |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | phosphorylated residue |
| Instrument | timsTOF Pro |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-02-06 15:18:52 | ID requested | |
| ⏵ 1 | 2026-04-01 01:00:33 | announced | |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Cofilin-1, MELK, Immunoprecipitation, Affinity Purification-MS, OTSSP167, HEK293T, T70 phosphorylation, LC-MS/MS |
Contact List
| Kuai Yu |
|---|
| contact affiliation | Guangzhou Medical University |
| contact email | yukuaic@126.com |
| lab head | |
| Kuai Yu |
|---|
| contact affiliation | Guangzhou Medical University |
| contact email | yukuaic@126.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD074164
- Label: PRIDE project
- Name: Identification of MELK-mediated Cofilin-1 T70 phosphorylation by IP-LC-MS/MS analysis
