Mouse Bone marrow-derived macrophages (BMDMs) and HT22 neuronal cells (subjected to 2-hour oxygen-glucose deprivation, OGD-2h) were used in this study. To investigate cell migration, a Transwell assay was performed. For pharmacological intervention, cells were treated with either the serotonin receptor 2B (5-HT2B) agonist BW723C86 or dimethyl sulfoxide (DMSO) as a vehicle control. Following treatment, global protein expression profiling was performed using mass spectrometry-based quantitative proteomics.