PXD074049

PXD074049 is an original dataset announced via ProteomeXchange.

Title	Recombinant dimeric PICK1 protein inhibitors for long-term relief of chronic pain by AAV therapeutics
Description	Chronic neuropathic pain’s impact, persistence, and limited treatments render it relevant for gene therapy. Here, we describe the development and application of self-assembling dimeric peptide inhibitors of the pain-associated scaffolding protein PICK1 (protein interacting with C-kinase 1) delivered by adeno-associated viral (AAV) vectors. In mice, these peptides prevented mechanical allodynia in inflammatory and neuropathic pain models and reversed neuropathic pain for up to one year. Targeting of somatosensory pain pathways relieved pain without overt side effects, while selective transduction of dorsal root ganglion (DRG) neurons was sufficient for providing pain relief. Using proteomic and phosphoproteomic analysis of DRG tissue, we identified protein kinase C alpha (PRKCA) substrate candidate, that potentially shape this pain-relieving phenotype. We also confirmed PICK1 expression and peptide target engagement in mice and human donor tissue, supporting the potential of AAV-based PICK1 inhibitors as a clinically meaningful strategy for neuropathic pain conditions.
HostingRepository	PRIDE
AnnounceDate	2026-04-10
AnnouncementXML	Submission_2026-04-10_01:55:34.125.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Antonio Marino
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	mono N-acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Ascend

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-04 03:44:42	ID requested
⏵ 1	2026-04-10 01:55:34	announced

Dataset with its publication pending

submitter keyword: PICK1

Protein interacting with C-kinase 1

neuropathic pain, pain treatment

neuropathic pain treatment

gene therapy

AAV therapeutics.

Andreas T. Sørensen
contact affiliation	Molecular Neuropharmacology and Genetics Laboratory, Department of Neuroscience, Faculty of Health and Medical Sciences, University of Copenhagen, 2200, Denmark
contact email	andreass@sund.ku.dk
lab head
Antonio Marino
contact affiliation	Proteomics Research Infrastructure (PRI) - UCPH
contact email	antonio.marino@pri.ku.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD074049

PRIDE project URI

• PRIDE
  1. PXD074049
     1. Label: PRIDE project
     2. Name: Recombinant dimeric PICK1 protein inhibitors for long-term relief of chronic pain by AAV therapeutics