The goal of this project was to observe proteomic differences between cancer cells surviving chemotherapy (1-, 5-, and 10-Days post-treatment removal) and untreated parental cells. This provides us with a top-down view of pathways that could be enriched/down-regulated throughout those timepoints when compared to untreated parental cells and allow us to generate hypotheses to start new projects. The PC3 and MDA-MB-231 cell lines were used in this study.