To identify Vibrio parahaemolyticus proteins that interact with TsrA, C-terminally 6ï‚´His-tagged TsrA (TsrA-6ï‚´His) expressed in Escherichia coli was immobilized on Ni-NTA beads and incubated with lysates from V. parahaemolyticus strain RIMD2210633. Proteins bound to TsrA were separated by SDS-PAGE, followed by Coomassie Brilliant Blue staining, and a protein of ~17 kDa was found to be specifically associated with TsrA. The protein band was subjected to liquid chromatography-tandem mass spectrometry.