Multiplex immunofluorescence staining for CD11b, CD168, CD8, and Pan-CK was performed on three colon cancer specimens to identify CCTR regions. Subsequently, CD8+ T cells located at varying distances from the CCTR were subjected to single-cell resolution laser capture micro-dissection (LCM) and spatial proteomics analysis. These cells were categorized into three groups: the CCTR group (≤50 μm, n=393), the ad-CCTR group (>50 μm and ≤200 μm, n=375), and the non-CCTR group (>200 μm, n=383).