PXD073018 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Treatment of human oocytes with extracellular vesicles from follicular fluid during rescue in vitro maturation enhances maturation rates and modulates oocyte proteome and ultrastructure |
| Description | This project aimed to elucidate whether human follicular fluid–derived extracellular vesicles (ffEVs) enhance oocyte maturation during rescue in vitro maturation (rIVM). We characterised the protein cargo of ffEVs isolated from follicles containing oocytes at different maturation stages (GV, GVBD, and MII), identifying distinct proteomic signatures associated with follicular competence and oocyte maturity. Subsequently, single-cell proteomic profiling of IVM-matured oocytes supplemented with ffEVs demonstrated that ffEV treatment induces significant changes in oocyte protein abundance, including factors linked to oocyte maturation, endoplasmic reticulum function, steroid biosynthesis, and cytoskeletal organisation. These proteomic alterations were accompanied by changes in oocyte ultrastructure and organelle architecture. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-04-06 |
| AnnouncementXML | Submission_2026-04-05_16:52:28.705.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Mara Saenz-de-Juano |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | iodoacetamide derivatized residue |
| Instrument | timsTOF Pro; Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-01-13 11:40:08 | ID requested | |
| ⏵ 1 | 2026-04-05 16:52:29 | announced | |
Publication List
| 10.1093/hropen/hoag021; |
| Makieva S, Saenz-de-Juano MD, Almi, ñ, ana C, Bauersachs S, Bernal-Ulloa S, Xie M, Velasco AG, Cervantes N, Sachs M, Ulbrich SE, Leeners B, maturation enhances maturation rates and modulates oocyte proteome and ultrastructure. Hum Reprod Open, 2026(2):hoag021(2026) [pubmed] |
Keyword List
| submitter keyword: follicular fluid, extracellular vesicles,Human oocyte, in vitro maturation, single-cell proteomic analysis |
Contact List
| Prof. Dr. Susanne Ulbrich |
|---|
| contact affiliation | ETH Zürich, Department of Environmental Systems Science |
| contact email | susanne.ulbrich@usys.ethz.ch |
| lab head | |
| Mara Saenz-de-Juano |
|---|
| contact affiliation | ETH Zurich |
| contact email | mara.saenz@usys.ethz.ch |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD073018
- Label: PRIDE project
- Name: Treatment of human oocytes with extracellular vesicles from follicular fluid during rescue in vitro maturation enhances maturation rates and modulates oocyte proteome and ultrastructure
