iPSC-derived retinal pigment epithelium (iPSC-RPE) cells were cultured in vitro under standard conditions. The cells were then subjected to either a control treatment or treatment with 200 µM ferric ammonium citrate (FAC) for a period of 10 days to induce iron overload. Following the treatment period, total cellular proteins were harvested from each group. Protein samples were subsequently prepared and analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) for global proteomic profiling. The experiment included two independent biological replicates for both the control and FAC-treated conditions to ensure statistical robustness.