Ascaridia galli (A. galli) is one of the most common intestinal nematodes of domesticated chickens and the cause of avian ascaridiasis, a globally important disease in poultry. Its genome is sparsely sequenced and its proteome is uncharacterized. We her investigate the proteome composition in larvae and in female and male worms of A. Galli, querying the LC-MS/MS data against a hybrid database of two related nematodes; Ascaris suum and Anisakis simplex. We compare the proteome of larvae, female and male worms.