The placenta is a highly dynamic organ that supports the growth and development of the fetus throughout the different stages of pregnancy. Malfunction of the placenta causes disorders of the pregnancy, including preeclampsia and pre-term birth. The study of the pathophysiology of the placenta at different stages of pregnancy has been hindered by limited access to the human placenta and lack of predictive cellular and animal models. Here, we describe 3D bioprinted vascularized human placenta barrier (hPB) tissue models using primary human trophoblasts and stroma cells that recapitulate the physiology of the human placenta at early and late stages of gestation. These bioprinted vascularized hPB tissue models mimic the architecture and function of late and early-stages human placenta, including barrier function, nutrient uptake, transporters activity and hormones secretion. These vascularized hPB models were assembled in a 96-well microplate format to enable robust drug screening for placenta diseases and safety.