Sprint interval exercise (SIE) induces mitochondrial adaptations in skeletal muscle, comparable to or greater than moderate-intensity continuous exercise (MICE), despite lower training volume. These effects are partly due to enhanced mitochondrial bioenergetics, including increased Ca²⁺ uptake and pyruvate dehydrogenase (PDH) activation. Oleuropein (OLE), a compound from olive leaf extract, also promotes mitochondrial Ca²⁺ uptake and PDH activation in mice. The current study tested whether OLE could enhance these adaptations in humans during MICE or SIE. In a double-blind crossover design, healthy males performed either MICE or SIE with OLE supplementation. To elucidate the acute molecular response of skeletal muscle following exercise, we performed mass spectrometry-based proteomic analysis.