Doxorubicin-induced cardiomyopathy (DiCM) is a leading cause of heart failure and mortality in cancer patients, with doxorubicin-induced cardiomyocyte apoptosis constituting a fundamental pathological mechanism. Cardiac resident macrophages are primarily responsible for the effective clearance of apoptotic cardiomyocytes (efferocytosis), a process pivotal for suppressing inflammatory response and adverse cardiac remodeling. To identify genes implicated in DiCM, RNA sequencing was performed using heart tissues from saline- and DOX-treated wild-type (WT) mice. Ubiquitin mass spectrometry was used to identify the ubiquitin site of nuclear receptor subfamily 1 group H member 2 (NR1H2).