To uncover the mechanism by which TBC1D23-E15 regulates erythropoiesis, we systematically compared the interactomes of long and short TBC1D23 isoforms through an integrated multi-omics approach. Firstly, we generated three K562 cell lines, one lacking TBC1D23 expression (KO) and the other two expressing only TBC1D23-L or TBC1D23-S, and performed immunoprecipitation in these cell lines followed by IP-MS