This project investigates the dynamic lung proteome in a murine model of lipopolysaccharide (LPS)-induced acute lung injury (ALI). C57BL/6N mice were exposed to LPS via intratracheal nebulization to induce ALI, and lung tissues were collected at multiple time points (non-stimulated controls, and days 1, 3, 7 and 14 after LPS challenge) to capture the onset, peak and resolution phases of lung injury.