⮝ Full datasets listing
PXD071614-1
PXD071614 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Co-translational targeting defect caused by Srp68 knockout leads tomeiotic arrest during spermatogenesis in mice |
| Description | Co-translational targeting by the signal recognition particle delivers nascent secretory and membrane proteins into the endoplasmic reticulum, yet its role in mammalian germ cells remains unclear. Here, we found that SRP68 is indispensable for spermatogenesis in mice. Conditional Srp68 deletion in germ cells results in defective synapsis and double-strand break repair, leading to meiotic prophase I arrest and male infertility. Proteomic profiling revealed that SRP68-deficient spermatocytes exhibit significant reduced endoplasmic reticulum (ER)-associated signal peptide-containing proteins, accompanied by structural disorganization. Additionally, multiple factors involved in ER–mitochondrial contact are decreased in SRP68-deficient spermatocytes, leading to mitochondrial Ca²⁺ depletion, depolarized membrane potential, and insufficient ATP production. These findings demonstrate that SRP68-dependent co-translational targeting is essential for meiotic progression, providing a basis for interpreting ER-related causes of male infertility. |
| HostingRepository | iProX |
| AnnounceDate | 2025-12-04 |
| AnnouncementXML | Submission_2026-03-31_17:55:30.953.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Hanzhen Li |
| SpeciesList | scientific name: Mus musculus; NCBI TaxID: 10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Fusion |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-12-04 22:39:18 | ID requested | |
| ⏵ 1 | 2026-03-31 17:55:31 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Meiosis, Co-translational,ER–mitochondria contacts,Male infertility |
Contact List
| Hongbin Liu | |
|---|---|
| contact affiliation | Shandong University |
| contact email | hongbin_sduivf@aliyun.com |
| lab head | |
| Hanzhen Li | |
| contact affiliation | Shandong University |
| contact email | lhzsdu8616@163.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
