This study is the first to report the effects of stimulation with recombinant ScGPA2, ScGPB5, and single-chain ScGPB5#ScGPA2 on vertebrate testicular tissue in vitro. A large-scale, untargeted proteomic analysis revealed for the first time that all three—ScGPA2, ScGPB5, and single-chain ScGPB5#ScGPA2—can modulate the abundance of 103 of the 7,619 identified proteins, including proteins related to spermiogenesis and immunity, as well as others that are less well characterized.