Updated project metadata. The processes such as DNA replication, transcription, and repair are often modulated by specific nuclear proteins, protein–protein interactions (PPIs), and post-translational modifications (PTMs). The Trypanosoma cruzi parasite is an interesting model to study these nuclear proteins and PPIs, especially involving histones and their post-translational modifications (hPTMs). However, the proteins and complexes that recognize and bind to them are poorly understood. In this work, we aimed to identify the PPIs of T. cruzi nucleus