Updated project metadata. Aim of this study was to compare proteomic profiles of the WT and the pMD8 mutant strains of Streptomyces albus. pMD8 is a mutant encoding a phosphoablative form of the CslA gene (T17A). Study is a part of a bigger project on the roles of PknB and CslA proteins in cell wall morphogenesis of Streptomyces. Another dataset related to this study is the PXD053764 phosphoproteomic analysis of the PknB deletion mutant strain vs the WT strain. This work was supported by Narodowe Centrum Nauki, 2020/39/D/NZ1/00303. The purchase of M-Class Acquity-Synapt XS LC–MS system was financially supported by the “Excellence Initiative – Research University” program for the University of Wroclaw, 2020–2025.