Many diseases, including diabetes and hypertension, can lead to kidney damage, often resulting in long-term health complications and organ failure. Both animal and human studies have documented a causal relationship between high dietary sodium intake and elevated blood pressure. We explored a proteomics analysis of plasma samples to detect early kidney damage in response to a high sodium (HS) diet through the quantification of putative kidney-derived proteins in plasma for early disease detection. Plasma samples from 7 female baboons fed a high sodium diet for 6 weeks were collected before and after the HS diet challenge. Plasma samples were analyzed using a novel nanoparticle enrichment protocol.