This dataset corresponds to the proteomic characterization of the TMEM160 interactome in A549 cells using co-immunoprecipitation (Co-IP) followed by data-independent acquisition mass spectrometry (DIA-MS). TMEM160 was immunoprecipitated using an anti-TMEM160 antibody, and interacting proteins were identified through LC–MS/MS analysis using a Q-Exactive HF-X system. Protein identification and quantification were performed in DIA-NN (v1.8.1) in library-free mode using the UniProt Human reference proteome.