Lysosomes were enriched from Wdr7 iKO MEFs stably expressing TMEM192-3xHA (Bait) or TMEM192-2xFlag (Ctrl) via lysosomal immunoprecipitation (Lyso-IP) using PierceTM anti-HA magnetic beads to analyze changes in the lysosomal protein content upon induced depletion of Wdr7.