Rising global temperatures are expected to increase the burden of infectious diseases, including the expansion of mosquito-borne illnesses into new regions. The Anopheles mosquito, the vector of malaria, is a highly sexually dimorphic species; only females require blood meals for egg production and thus transmit disease. Understanding the molecular basis of sex differences in mosquitos may contribute to the design of novel tools for controlling disease transmission. Here, we established baculovirus as a gene delivery tool in female Anopheles cells to investigate the cellular effects after ectopic expression of Yob, the male-determining gene and upstream regulator of sex-specific pathways.