Egg PVF proteins have been the primary focus in the water-to-land transition of ampullariids over the last three decades, but there is a lack of PVF data in Old-World species. To better understand the function and evolutionary history of PVF proteins, we generated Old-World Pila and Lanistes PVF proteomes. It also supplements the data on Ovorubin of Pomacea canaliculata, further helping to confirm the subunit of PV1 in PVF protein.