This dataset corresponds to the proteomic analysis presented in Figure 6A of the manuscript “Obesity-driven microbial GABA depletion promotes metabolic rewiring and colorectal cancer progression.” We performed Drug Affinity Responsive Target Stability (DARTS) and co-immunoprecipitation proteomics using colorectal cancer cell lysates incubated with glyceraldehyde-3-phosphate (GAP) to identify GAP-binding proteins. Mass spectrometry data revealed PPP1CA as a key GAP-interacting protein mediating GABA-dependent suppression of YAP signaling. The dataset supports the mechanistic link between microbiota-derived GABA, TPI1-generated GAP, and YAP-regulated tumor metabolism in obesity-associated colorectal cancer.