Diabetic cardiomyopathy (DbCM) halts cardiac performance with a complex etiology, hence the absence of an effective therapy for this disease. Herein we decoded the proteins hub interconnected with Calmodulin (CaM), a striatin (STRN) binding protein, that plays a role in DbCM. Using CaM as a bait, we precipitated protein clusters from left ventricles (LVs) of diabetic hearts at two stages of diabetes; 8- and 24-weeks rats treated with streptozotocin-STZ. Diabetic rats showed pathological heart remodeling confirmed by increased heart/body weight (HW/BW) ratio and increased β-MHC protein levels and ANF mRNA expression.