High yield and wide adaptation of rice is of significance for more grain supply and food security. Here, we report a type II phosphatidylinositol 4-kinase, PI4Kγ7, harboring a PI3/PI4 kinase domain, which positively regulates rice yield and affords wide adaptation of rice. We show that PI4Kγ7 interacts with a CCCH-type zinc finger transcription factor, OsLIC. Further investigations discover that PI4Kγ7 modulates rice yield by facilitating the stability and cytoplasm-to-nucleus translocation of OsLIC through phosphorylation. To identify the residue(s) being phosphorylated by PI4Kγ7, we performed a liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis