To provide comprehensive characterization of the cardiac proteome of advanced human heart failure, we performed mass spectrometry-based label free proteomic analysis of left ventricular samples obtained from 149 human heart failure patients and 9 non-failing controls. All heart failure biopsy samples were collected at the time of heart transplantation or assist device implantation. To characterize signaling perturbations, we performed phosphoproteomic analysis for 32 representative heart failure samples and 8 non-failing controls.