Pancreatic ductual adenocarcinoma (PDAC) is a highly metastatic and chemo-resistant disease. Proteins secreted by cancer cells are involved in cell-cell communications and matrix remodeling and play an important role in modulating the tumor microenvironment. The purpose of this study was to establish the secretion profiles of PDAC primo-culture cell lines and use this data to identify secretion signatures that could correlate with biological and/or clinical parameters.