Although tissue engineering studies have proven the feasibility of “chondrocyte-seeded scaffolds” for the formation of cartilaginous tracheal grafts in vivo, the use of these scaffolds is highly limited in humans owing to the scarcity of young chondrocytes and the dedifferentiation of in vitro expanded chondrocytes. In this study, we proposed a clinically operable approach to fabricate vascularized tracheal grafts through minced cartilage tissues and allogenous adipose derived mesenchymal stem cells and examined their behavior in tracheal defects in rabbit and porcine models. Using a template-sacrificing approach, we fabricated 3D-shaped (PCL-PGS) minichannels to enable the injection of "adipose stem cells - cartilage granules - platelet-rich plasma" constructs, while allowing nutritional perfusion from the wrapped fascia. Two weeks after ectopic transplantation, the grafts became vascularized and exhibited mechanical strength comparable to that of the native trachea. These grafts were subsequently anastomosed end-to-end to tracheal defects in both the rabbit and porcine models. Histological analysis revealed that the 3D-shaped "CG-ADSCs-PRP" compounds reassembled into cartilage rings and integrated with vascularized fascia, supporting animal survival for up to 67 days. ADSCs significantly enhanced the survival of cartilage granules within the morphed channels, primarily through increased glycolysis in chondrocytes.