To investigate the impact of Toxoplasma gondii GRA35 on neuronal cells and the underlying mechanisms, we conducted GST-pull down experiments to isolate proteins that bind to GRA35-GST and GST in neuronal cells, respectively. The dataset labeled "20240706_WJ_ GRA35_N_B01.raw" corresponds to the GRA35-GST group, whereas "20240706_WJ_C1_B01.raw" represents the data for the GST group. Subsequent mass spectrometry analysis revealed that the neuron-specifically expressed RTN1c protein interacts with GRA35.