Plant flowering represents a critical transition from vegetative growth to reproductive growth and serves as an important marker of plant developmental maturity. The flowering repressor gene FLC is a key regulator of floral transition. Although extensive research has been conducted on FLC, the mechanisms governing FLC mRNA degradation and translational regulation remain unclear. In previous studies, we identified the GYF family by screening regulatory factors near the nuclear pore complex (NPC). We found that GYF proteins are enriched near the nuclear membrane and can interact with components of both the NPC and processing bodies (P-bodies). Functional studies revealed that gyf mutants exhibit a late-flowering phenotype, primarily due to elevated FLC mRNA levels. In vitro and in vivo biochemical experiments demonstrated that GYF proteins directly bind to the 5' UTR and 3' UTR of FLC mRNA, cooperate with 4EHP to participate in translation-coupled mRNA degradation and translational repression of FLC.