PXD067510

PXD067510 is an original dataset announced via ProteomeXchange.

Title	The inner nuclear membrane protein LEMD3-interacting proteins
Description	HEK293T cells were transfected with the pcDNA3.1 or LEMD3-FLAG plasmid.Forty-eight hours after transfection, the cells were lysed in lysis buffer (50 mM Tris-HCl [pH=7.4], 150 mM NaCl, 2 mM EDTA, and 0.3% NP-40). The cell lysate samples were incubated with anti-FLAG M2 affinity gel (A2220, Sigma‒Aldrich) according to the manufacturer’s instructions. The anti-FLAG M2 affinity gel-bound proteins were eluted with FLAG peptide (F3290, Sigma). The final eluent was collected and resolved on an SDS-PAGE gel. The gel was fixed with a 30% ethanol:10% acetic acid solution, washed with 10% ethanol, and then subjected to LC‒MS/MS. Samples from HEK293T cells transfected with the pcDNA3.1 plasmid were used as the negative control.
HostingRepository	iProX
AnnounceDate	2025-08-19
AnnouncementXML	Submission_2025-08-19_20:04:16.009.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	WenqiangLi
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-19 20:03:48	ID requested
⏵ 1	2025-08-19 20:04:16	announced

Dataset with its publication pending

submitter keyword: LEMD3

Wei Kong
contact affiliation	Department of Physiology & Pathophysiology, Peking University Health Science Center
contact email	kongw@bjmu.edu.cn
lab head
WenqiangLi
contact affiliation	Department of Physiology & Pathophysiology, Peking University Health Science Center
contact email	lwq@bjmu.edu.cn
dataset submitter

iProX dataset URI