PXD067425

PXD067425 is an original dataset announced via ProteomeXchange.

Title	TurboID-mediated proximal labeling of Drosophila executioner caspases, Dcp-1 and Drice, in wing imaginal discs
Description	V5-TurboID was knock-in tagged in Drosophila executioner caspases, Dcp-1 and Drice, which are expressed in wing imaginal discs. Flies were fed with 100 µM biotin for protein biotinylation in two biological replicates. Biotinylated protein-containing lysates extracted from wing imaginal discs were subjected to FG-NeutrAvidin beads purification followed by on-beads trypsin digestion. Digested peptides were desalinated and purified using GL-tip SDB. Purified peptides were subjected to LC-MS/MS analysis.
HostingRepository	jPOST
AnnounceDate	2026-08-18
AnnouncementXML	Submission_2026-08-17_08:00:14.101.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Natsuki Shinoda
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	L-cysteine methyl disulfide; alpha-amino acetylated residue; L-methionine sulfoxide; ubiquitination signature dipeptidyl lysine
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-17 22:57:59	ID requested
⏵ 1	2026-08-17 08:00:14	announced

Dataset with its publication pending

submitter keyword: Dcp-1, Drice, Caspase, Drosophila, TurboID, LFQ

Masayuki Miura
lab head
Natsuki Shinoda
contact affiliation	The University of Tokyo
dataset submitter

jPOST dataset URI

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