Extracellular vesicles are bubbles used by cells for intercellular and intertissue interactions. Their cargo includes various proteins, nucleic acids, peptides, which, interacting with cells, trigger intracellular cascades. Proteomic analysis of extracellular vesicles from different cell types has already been performed. Here, we present the first proteomic analysis of extracellular vesicles proteins obtained from three lines of glial progenitor cells differentiated from human induced pluripotent stem cells.