PXD067104

PXD067104 is an original dataset announced via ProteomeXchange.

Title	Multi-layered metabolic remodeling of Pseudomonas putida for efficient conversion of lignocellulosic sugars to a precursor of advanced aviation fuel
Description	Isoprenyl acetate, a volatile ester derived from isoprenol, is a key biosynthetic intermediate for a candidate for next-generation aviation fuel, 1,4-dimethylcyclooctane. Here, we engineered Pseudomonas putida KT2440 for the production of isoprenyl acetate from mixed sugar substrates. We first generated isoprenyl acetate by introducing a heterologous alcohol acetyltransferase (ATF1) and deleting three promiscuous native esterases to reduce product degradation. Then, we engineered efficient glucose and xylose co-utilization by integrating a heterologous xylose isomerase pathway and deleting global regulators crc and hexR to alleviate catabolite repression. Additionally, intracellular acetyl-CoA flux was reinforced through the expression of auxiliary carbon-conserving routes, including non-oxidative glycolysis and acetate assimilation. Culture conditions were systematically optimized by adjusting media composition, induction, and overlay solvent to maximize product yield. These cumulative efforts led to isoprenyl acetate titers of 1.5 g/L in shake flasks and 1.9 g/L in fed-batch fermentor cultures from mixed sugars, corresponding to a yield of 0.067 g/g of total sugar consumed. Our work demonstrates the potential of P. putida as a robust microbial chassis for scalable biosynthesis of ester-based biofuels from lignocellulosic feedstocks.
HostingRepository	PanoramaPublic
AnnounceDate	2025-08-07
AnnouncementXML	Submission_2025-08-07_17:36:15.689.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Chris Petzold
SpeciesList	scientific name: Pseudomonas putida KT2440; NCBI TaxID: 160488;
ModificationList	Carbamidomethyl
Instrument	6460 Triple Quadrupole LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-07 17:30:56	ID requested
⏵ 1	2025-08-07 17:36:16	announced

no publication

submitter keyword: Isoprenyl acetate, isoprenol, advanced aviation fuel, catabolite repression, lignocellulosic feedstocks, esterase knockout

Chris Petzold
contact affiliation	Lawrence Berkeley National Laboratory
contact email	cjpetzold@lbl.gov
lab head
Chris Petzold
contact affiliation	Lawrence Berkeley National Laboratory
contact email	cjpetzold@lbl.gov
dataset submitter

Panorama Public dataset URI