TTC33 is a 30 kDa human uncharacterized tetratricopeptide repeat protein. Here, we employed label-free mass spectrometry analysis to identify the proteins interactors. The TTC33-associated network (TAN) is composed of WDR61, PHF5A, which together with TTC33 form a trimeric core complex (TANC), that recruits UNG1/2 DNA glycosylase, uncharacterized CCDC97, SF3B, a sub-complex of U2-splicing factor, and PP2A phosphatase (PPP2R1A, PPP2CA/CB and PPP2R2A).