PXD066845-1

PXD066845 is an original dataset announced via ProteomeXchange.

Title	Reduction in synaptic vesicle proteins but increase in proteins involved in basic cell function in cerebral cortices without the endosomal SNARE proteins Vti1a and Vti1b
Description	Absence of the endosomal SNAREs vti1a and vti1b results in perinatal death and severe neuronal phenotypes in mice while lack of one of these proteins results in minor phenotypes. Proteomic differences were investigated to obtain a deeper insight into processes in which Vti1a and Vti1b are involved. Here we applied a bottom-up shotgun proteomic approach to investigate the differences in wild-type, double heterozygous (DHET),vti1a vt1ib double knockout (DKO), vti1a knockout and vti1b knockout cerebral cortices. Single deletions did not affect protein levels. A total of 1725 proteins were detected of which 69 were down and 191 proteins were upregulated in DKO cortices. Many less abundant proteins belonged to the gene ontology terms and Reactome pathways synapse, synaptic vesicle cycle, vesicle mediated transport and L1CAM interaction in pathway enrichment analysis. More abundant proteins were enriched in Reactome- and KEGG-pathways such as spliceosome, ribosome, carbon metabolism and gene expression. Immunoblotting validated reduced expression levels of the tested synaptic vesicle proteins as well as increased amounts of lysophosphatidylcholine acyltransferase 1 (Lpcat1) and neuron-specific gene 2 (Nsg2 ), which is involved in postsynaptic AMPA-receptor recycling. These data indicate that the synapse and cell adhesion were strongly affected in DKO brains.
HostingRepository	PRIDE
AnnounceDate	2026-03-31
AnnouncementXML	Submission_2026-03-31_11:11:26.520.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Julia Voss
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-31 23:52:19	ID requested
⏵ 1	2026-03-31 11:11:27	announced

Gottschalk J, Kotschnew K, Hahn J, Patschkowski T, von Fischer von Mollard G, Reduction in Synaptic Vesicle Protein Abundance but Increased Amounts of Nsg2 and Lpcat1 in Cerebral Cortices Without the Endosomal SNARE Proteins Vti1a and Vti1b. Proteomics, 26(5):86-97(2026) [pubmed]

10.1002/pmic.70117;

submitter keyword: membrane traffic, SNARE,Endosome, neurodegeneration, proteome

Gabriele Fischer von Mollard
contact affiliation	Biochemistry III, Department of Chemistry, Bielefeld University, Universitätsstr. 25, 33615 Bielefeld, Germany
contact email	gabriele.mollard@uni-bielefeld.de
lab head
Julia Voss
contact affiliation	Bielefeld University, Center for Biotechnology
contact email	juvoss@cebitec.uni-bielefeld.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD066845
     1. Label: PRIDE project
     2. Name: Reduction in synaptic vesicle proteins but increase in proteins involved in basic cell function in cerebral cortices without the endosomal SNARE proteins Vti1a and Vti1b