PXD066703
PXD066703 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | The Neurolipid Atlas: a lipidomics resource for neurodegenerative diseases uncovers cholesterol as a regulator of astrocyte reactivity impaired by ApoE4 |
| Description | Lipid changes in the brain have been implicated in many neurodegenerative diseases including Alzheimers Disease (AD), Parkinsons disease and Amyotrophic Lateral Sclerosis. To facilitate comparative lipidomic research across brain-diseases we established a data commons named the Neurolipid Atlas, that we have pre-populated with novel human, mouse and isogenic induced pluripotent stem cell (iPSC)-derived lipidomics data for different brain diseases. We show that iPSC-derived neurons, microglia and astrocytes display distinct lipid profiles that recapitulate in vivo lipotypes. Leveraging multiple datasets, we show that the AD risk gene ApoE4 drives cholesterol ester (CE) accumulation in human astrocytes recapitulating CE accumulation measured in the human AD brain. Multi-omic interrogation of iPSC-derived astrocytes revealed that cholesterol plays a major role in astrocyte interferon-dependent pathways such as the immunoproteasome and major histocompatibility complex (MHC) class I antigen presentation. We show that through enhanced cholesterol esterification ApoE4 suppresses immune activation of astrocytes. Our novel data commons, available at neurolipidatlas.com, provides a user-friendly tool and knowledge base for a better understanding of lipid dyshomeostasis in neurodegenerative diseases. |
| HostingRepository | MassIVE |
| AnnounceDate | 2025-07-29 |
| AnnouncementXML | Submission_2025-07-29_01:54:47.918.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Leibniz FLI Proteomics |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Carbamidomethyl; Acetyl; Oxidation |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-07-29 00:22:36 | ID requested | |
| ⏵ 1 | 2025-07-29 01:54:48 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: ApoE4, neurolipids, Alzheimer Disease (AD), Parkinson disease, Amyotrophic Lateral Sclerosis, DatasetType:Proteomics |
Contact List
| Rik van der Kant | |
|---|---|
| contact affiliation | Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, |
| contact email | r.h.n.vander.kant@vu.nl |
| lab head | |
| Leibniz FLI Proteomics | |
| contact affiliation | Leibniz FLI |
| contact email | proteomics@leibniz-fli.de |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v10/MSV000098665/ |
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