PXD066679

PXD066679 is an original dataset announced via ProteomeXchange.

Title	Abnormal mitochondrial structure and function in brown adipose tissue of SLC35A4-MP knockout mice
Description	Uncovering the role of upstream ORFs (uORFs) challenges conventional views of one protein per mRNA while also revealing the capacity of some uORFs to encode microproteins that contribute to cellular biology and physiology. This study explores the functional role of a recently identified mitochondrial microprotein, SLC35A4-MP, in the brown adipose tissue of mice. Our findings reveal dynamic regulation of SLC35A4-MP expression during primary brown adipocyte differentiation in vitro and during cold exposure or high-fat diet (HFD)-induced obesity in mice. Using a knockout mouse model, we show that loss of SLC35A4-MP disrupts mitochondrial lipid composition, decreasing cardiolipins and phosphatidylethanolamine in brown adipose tissue from HFD-fed mice. SLC35A4-MP deficiency also impairs mitochondrial activity, alters mitochondrial number and morphology, and promotes inflammation. For example, knockout mice accumulate acylcarnitines during cold exposure, indicating defective fatty acid oxidation. These findings reveal SLC35A4-MP as a previously unrecognized microprotein in the endogenous regulation of mitochondrial function and tissue lipid metabolism, adding to the growing list of functional endogenous microproteins
HostingRepository	PRIDE
AnnounceDate	2025-07-29
AnnouncementXML	Submission_2025-07-29_08:40:59.461.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Eduardo Vieira de Souza
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-28 13:04:49	ID requested
⏵ 1	2025-07-29 08:40:59	announced

Dataset with its publication pending

submitter keyword: uORFs,microproteins, SLC35A4, upstream ORFs

Alan Saghatelian
contact affiliation	Salk Institute for Biological Studies
contact email	asaghatelian@salk.edu
lab head
Eduardo Vieira de Souza
contact affiliation	Salk Institute for Biological Studies
contact email	esouza@salk.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD066679
     1. Label: PRIDE project
     2. Name: Abnormal mitochondrial structure and function in brown adipose tissue of SLC35A4-MP knockout mice